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ViewRNATM ISH Cell Assay for Fluorescence RNA In Situ Hybridization (RNA FISH)

Fluorescence RNA In Situ Hybridization (RNA FISH)—Overview

Fluorescence based in situ hybridization (FISH) techniques are used to visualize DNA or localized RNAs within cells. However the fluorescence in situ hybridization of RNA has always been limited by low sensitivity, complicated workflow and the inability to perform multiplex analysis. ViewRNA is a novel non-radioactive RNA in situ hybridization solution that offers single-molecule RNA sensitivity for one to four RNA targets. Affymetrix Research-Use-Only (RUO) products for RNA FISH analysis allow investigators doing biomarker discovery or clinical research to validate and study RNA biomarkers in suspension or adherent cells.

ViewRNA ISH Cell Assay—Unique Benefits

  • Sensitive in situ hybridization assay – detect one mRNA molecule/cell
  • Multiplex in situ hybridization – study one to four mRNA targets per cell
  • Flexible probe set design – to target any mRNA sequence
  • Fast results – one day assay
  • Fluorescence signal – use of fluorescence microscope (no radioactivity involved)
  • Robust, reproducible results in adherent or suspension cells

ViewRNA ISH Cell Assay—Applications

  • Analysis of transcriptional heterogeneity
  • Cancer and other disease research
  • Biomarker validation
  • Neurobiology/neuroscience
  • Infectious diseases/virology
  • Stem cells and differentiation research
  • RNAi knockdown quantitation
  • Study of non-coding RNAs
  • A companion to immunofluorescence (IF), immunocytochemistry (ICC) or DNA FISH  assays

Assay Performance Highlights

ViewRNATM ISH Cell Assay

Sample Type Adherent and suspension cells*
Species Any
Limit of Detection (LOD) 1 RNA molecule/cell
Plex Level 1 to 4-plex
Assay Format Slides, 4-well chamber, 24-well plates (cover slips)
Detection Fluorescent
Automation-compatible Fluorescence microscope or imaging system

* The ViewRNA ISH Cell Assay for multiplex fluorescence RNA in situ hybridization, has been optimized for analyzing adherent and suspension cultured cells. However other samples may be used - like circulating tumor cells, cells isolated from blood or other body fluids. We do not provide a protocol for the analysis of target RNAs from those starting materials. However we do recommend that you follow the protocol for analyzing suspension cells and you optimize as necessary.