Affymetrix | Panomics Solutions
Quantitative Biology Delivered.

miRNA Expression Assay: Overview

miRNA (microRNA) Assay - Unique Benefits

  • Functionally validated microRNA probe sets
  • Direct from lysate quantitation – no biases associated with microRNA isolation or amplification
       o Lyse and go sample preparation
       o Simplified workflow
       o Improved microRNA recovery, accuracy & precision (miRNA isolation step – can lose up to 50% of miRNA population)
  • High specificity towards the target microRNA and not to other family members
  • Excellent results in a variety of sample including blood, plasma, fresh & FFPE tissues, plant tissues, cultured cells and isolated miRNA
  • Quantitation using synthetic miRNA control or housekeeping gene
  • Excellent Concordance with New GeneChip® miRNA 2.0 Array

miRNA (microRNA) Probe Sets - Functionally Validated

  • Each probe set is validated for specificity & sensitivity (limit of detection)
  • High specificity to target miRNA and not to other family members
  • Probe set recognize ONLY mature miRNA

miRNA (microRNA) Assay - Applications

  • Validation of miRNA array results
  • Quantitation of miRNA from clinical samples
  • Study of miRNA regulation in cancer and other disease states

Direct from Lysate miRNA Expression Profiling

miR-31, miR-34a, miR-145, miR-181a and miR-222 were analyzed using the QuantiGene 2.0 miRNA Assay on RNAs purified from 16,000 HeLa cells using Ambion mirVana miRNA Isolation Kit and on cell lysates of the same number of cells. The microRNA expression results were normalized to the direct lysate data for each miRNA assay. Data indicated that signal of the test miRNAs in cell lysates was 1.8 fold higher than purified RNAs, and that miRNAs are lost during the purification step leading to purification biases in the profiling assay. 


Figure1. Comparison of miRNA profiles in purified RNA and cell lysate from 16,000 HeLa cells.

Assay Specifications

Cross Variation
≤ 10%
Sensitivity > 3,000 miRNA copies/assay well
Specificity One base
Dynamic Range 3 logs
Probe Length 25-mer; less, if miRNA length is less than 25